Discrimination of single-point mutations in unamplified genomic DNA via Cas9 immobilized on a graphene field-effect transistor

Discrimination of single-point mutations in unamplified genomic DNA via Cas9 immobilized on a graphene field-effect transistor
Nat Biomed Eng. 2021 Apr 5. doi: 10.1038/s41551-021-00706-z. Online ahead of print.ABSTRACTSimple and fast methods for the detection of target genes with single-nucleotide specificity could open up genetic research and diagnostics beyond laboratory settings. We recently reported a biosensor for the electronic detection of unamplified target genes using liquid-gated graphene field-effect transistors employing an RNA-guided catalytically deactivated CRISPR-associated protein 9 ... read more
Source: PubMedPublished on 2021-04-06By Sarah Balderston